Using urine test strips

Intestinal bacteria convert the conjugated bilirubin that using urine test strips excreted by the bile duct into the intestine into urobilinogen and stercobilinogen. False-negative reactions can result when urine with a high specific gravity contains crenated red blood cells that do not lyse when they come in contact with the reagent pad. It is possible to use an ammonia sulphate precipitation test in order to distinguish between hemoglobinuria and using urine test strips. In the range 5 to 9 the strips show colours that change from orange at pH 5, passing through yellow and green to dark blue at pH 9.

Using urine test strips
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Urine test strip

The urine test strip reaction is based on the action of leukocyte esterase in catalysing the hydrolysis of an ester of indolecarboxylic acid. Hematuria produces cloudy red urine, and hemoglobinuria appears as a clear red specimen. Automated readers of test strips also provide results using units from the International System of Units. The pad also includes bromothymol blue which measures this change in pH. The nitrite test is not particularly reliable and negative results in the presence of clinical symptoms are not uncommon, meaning that the test should not be taken as conclusive.

Using urine test strips
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Any deterioration in liver function reduces its ability to process the recirculated urobilinogen. It is normal to find up to 3 occasionally 5 leukocytes per high power field 40X in a urine sample, with women having slightly higher results owing to vaginal contamination. Urine Specific Gravity.

Using urine test strips
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Using urine test strips

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